Dr. Melissa Bredow

Postdoctoral Fellow

Melissa Bredow, Ph.D.

11mb95@queensu.ca

 

 

EDUCATION

2012-2017: PhD Biology, Queen’s University

2006-2011: BSc Honors Specialization in Cell and Developmental Biology & Minor Genetics, University of Western Ontario

RESEARCH INTEREST

My research seeks to understand the molecular underpinnings governing plant adaptation to biotic and abiotic stresses.

During my PhD, I studied freeze-tolerance mechanisms in the perennial ryegrass Lolium perenne and the annual model grass Brachypodium distachyon. This work focused on a specialized family of protective proteins known as ice-binding proteins (IBPs), that have a unique ability to restrict intercellular ice-crystal crystal growth at sub-zero temperatures. Using genetic and biochemical tools, I explored how IBPs protect cells from both freezing damage and infection associated with certain psychrotolerant microbial pathogens.

My current research focuses on immune homeostasis in the model plant Arabidopsis thaliana, working with Dr. Jacqueline Monaghan. This research aims to understand the mechanisms governing an appropriate immune response, incumbent to the generation of crops with improved pathogen resistance without associated growth or developmental trade-offs. Current research includes the identification of novel immune regulators through forward and reverse genetics screens and understanding how post-translational modifications can influence the activity of immune proteins.

PUBLICATIONS

BREDOW, M., and Monaghan, J. Calcium-dependent protein kinases in plant innate immunity. (In progress)

BREDOW, M., Juurakko, C., Hiroyuki, I., Nakayama, T., Kawamura, Y., Uemura, M., and Walker, V.K. Freeze and disease resistance proteins associated with the plasma membrane of cold-acclimated Brachypodium distachyon. (In progress)

BREDOW, M., and Walker, V. (2017) Ice-binding proteins in plants. Frontiers in Plant Science, DOI:  https://doi.org/10.3389/fpls.2017.02153.

BREDOW, M., Tomalty, H., and Walker, V.K. (2017) Identification of plant ice-binding proteins through assessment of ice-recrystallization inhibition and isolation using ice-affinity purification. Journal of Visualized Experiments, DOI: 10.3791/55302.

BREDOW, M., Smith, L., Tomalty, H., and Walker, V.K. (2017) Ice and anti-nucleating activity of an ice-binding protein form the annual grass, Brachypodium distachyon. Plant, Cell & Environment, DOI: 10.111/pce.12889.

BREDOW, M., Vanderbeld, B. and Walker, V.K. (2016) Knockdown of ice-binding proteins in Brachypodium distachyon demonstrates their role in freeze protection. PLoS ONE, 11(12):e016794.

BREDOW, M., Vanderbeld, B., and Walker, V.K. (2016) Generation and characterization of an ice-binding protein knockdown in a model crop. Cryobiology, 73(3): 420-421.

BREDOW, M., Vanderbeld, B., and Walker, V.K. (2016) Ice-binding proteins confer freezing tolerance in transgenic Arabidopsis thaliana. Plant Biotechnology Journal, DOI: 10.1111/pbi.12592.

Middleton, A. J., Vanderbeld, B., BREDOW, M., Tomalty, H., Davies, P. L., and Walker, V. K. (2014) Isolation and characterization of ice-binding proteins from higher plants. Methods in Molecular Biology, Chapter 19: Plant Cold Acclimation.

ORAL Presentations

2018: Invited keynote speaker: 11th International Cold Hardiness Meeting (Wisconsin, USA)

BREDOW, M., and Walker, V.K. ‘Ice-binding proteins: a protective mechanism against freezing and bacterial ice-nucleation’

2018: International Plant Immunity Symposium (Goettingen, Germany)

BREDOW, M., and Monaghan, J. ‘Regulation of immune homeostasis in Arabidopsis thaliana’

2018: Zentrum für Molekularbiologie der Pflanzen Departmental Seminar (Tuebingen, Germany)

BREDOW, M., and Monaghan, J. ‘Regulation of immune homeostasis in Arabidopsis thaliana’

2017: Departmental Seminar (Queen’s University, Kingston Ontario)

BREDOW, M., ‘Characterization and knockdown of a family of ice-binding proteins from freeze-tolerant grasses’

2016: The 53rd Annual Meeting of the Society for Cryobiology (Fairmont Hotel, Ottawa Ontario)

BREDOW, M., Vanderbeld, B., and Walker, V.K. ‘Generation and characterization of ice-binding protein knockdown in a model crop’

2016: Plant Biotechnology Conference (Queen's University, Kingston Ontario)

BREDOW, M., Vanderbeld, B., and Walker, V.K. ‘Apoplastic localization of ice-recrystallization inhibition proteins confer freezing tolerance to Arabidopsis thaliana’

2015: Japan Society for the Promotion of Science International Conference (Tokyo, Japan)

BREDOW, M., Umeura, M., and Walker, V.K. ‘Do antifreeze proteins associate with the plasma membrane of cereal grasses during cold-acclimation?’

2014: Graduate Student Research Day (Queen's University, Kingston Ontario)

BREDOW, M., and Walker, V.K. ‘Elucidating the mechanisms underlying antifreeze protein induced freezing tolerance in plants’

2014: Plant Biotechnology Conference (McGill University, Montreal)

BREDOW, M., Vanderbeld, B., and Walker, V.K. ‘Ice-recrystallization inhibition proteins from Lolium perenne confer freezing tolerance to Arabidopsis’

2013: Molecular & Cellular Biology Seminar Series (Queen's University, Kingston Ontario)

BREDOW, M. ‘Synthetic Biology’

GUEST LECTURES

2018: Biology 506, Biochemical Adaptations of Life Under Extreme Conditions (Queen's University, Kingston Ontario)

BREDOW, M. ‘Ice-binding proteins and natural freezing tolerance mechanisms’

2017: Biology 440, Biotechnology (Queen's University)

BREDOW, M. ‘Generation of transgenic plants for improved cold-hardiness’

2015: Biology 506, Biochemical Adaptations of Life Under Extreme Conditions (Queen's University, Kingston Ontario)

BREDOW, M. ‘Ice-binding proteins and natural freezing tolerance mechanisms’

2014: Biology 440, Biotechnology (Queen's University)

BREDOW, M. ‘Generation of transgenic plants for improved cold-hardiness’

POSTER PRESENTATIONS

2013: Meeting of the Canadian Society of Plant Biologists (Laval University, Quebec, Ontario)

BREDOW, M., Vanderbeld, B., Embleton L., and Walker, V.K. ‘Characterization of ice-binding proteins from the temperate grass Brachypodium distachyon

I've spent more time than many will believe [making microscopic observations], but I've done them with joy, and I've taken no notice those who have said why take so much trouble and what good is it?

Antonie van Leeuwenhoek

It's a parts list... If I gave you the parts list for the Boeing 777 and it had 100,000 parts, I don't think you could screw it together and you certainly wouldn't understand why it flew

Eric Lander

What is true for E. coli is also true for the elephant

Jacques Monod

The world becomes full of organisms that have what it takes to become ancestors. That, in a sentence, is Darwinism

Richard Dawkins

Shall we conjecture that one and the same kind of living filaments is and has been the cause of all organic life?

Erasmus Darwin

Nature proceeds little by little from things lifeless to animal life in such a way that it's impossible to determine the line of demarcation

Aristotle

Cells let us walk, talk, think, make love, and realize the bath water is cold

Lorraine Lee Cudmore

In the distant future I see open fields for far more important researches. Psychology will be based on a new foundation, that of the necessary acquirement of each mental power and capacity by gradation. Light will be thrown on the origin of man and his history

Charles Darwin

It is my belief that the basic knowledge that we're providing to the world will have a profound impact on the human condition and the treatments for disease and our view of our place on the biological continuum

J. Craig Venter

Imagine a house coming together spontaneously from all the information contained in the bricks: that is how animal bodies are made

Neil Shubin

A grain in the balance will determine which individual shall live and which shall die - which variety or species shall increase in number, and which shall decrease, or finally become extinct

Charles Darwin

The stuff of life turned out to be not a quivering, glowing, wondrous gel but a contraption of tiny jigs, springs, hinges, rods, sheets, magnets, zippers, and trapdoors, assembled by a data tape whose information is copied, downloaded and scanned

Steven Pinker

We wish to discuss a structure for the salt of deoxyribose nucleic acid. (D.N.A.). This structure has novel features which are of considerable biologic interest

Rosalind Franklin

We are biology. We are reminded of this at the beginning and the end, at birth and at death. In between we do what we can to forget

Mary Roach

The systems approach to biology will be the dominant theme in medicine

Leroy Hood

I've always been interested in animal behavior, and I keep reading about it because it's so surprising all the time - so many things are happening around us that we neglect to look at. Part of the passion I have for biology is based on this wonderment"

Isabella Rossellini

Because all of biology is connected, one can often make a breakthrough with an organism that exaggerates a particular phenomenon, and later explore the generality

Thomas Cech

Nothing in Biology makes sense except in the light of evolution

Theodosius Dobzhansky

Biology is now bigger than physics, as measured by the size of budgets, by the size of the workforce, or by the output of major discoveries; and biology is likely to remain the biggest part of science through the twenty-first century

- Freeman Dyson

Nothing can be more incorrect than the assumption one sometimes meets with, that physics has one method, chemistry another, and biology a third

- Thomas Huxley